1. Meafaigaluega masani
1. Meafaigaluega i le potu sauniuni
Tu'u vai fa'asusu ta'itasi, fa'asuavai fa'alua fa'afefeteina, tane vai, umu, kuka fa'amalosi, kapoti teu (teu mea e le'i fa'amama), kapoti teu (teu mea fa'ama'i), laulau afifi.Meafaigaluega i totonu o le potu sauniuni fofo: paleni torsion ma paleni faaeletonika (fuainumera vailaau), PH mita (fua PH taua o le vaifofo aganuu), stirrer mageta (faatulaga le potu fofo e fafagu fofo).
2. Meafaigaluega o le potu faaleaganuu
tane nitrogen vai, kapoti teu oloa (teu mea eseese), moli felanulanua'i ma lamepa ultraviolet, faiga e faamama ai le ea, pusaaisa maualalo le vevela (- 80 ℃), fa'a'ea malulu, fa'aigoa carbon dioxide, laulau pito (tusi fa'amaumauga o su'ega).
3. Meafaigaluega e tatau ona tuʻu i totonu o le potu faʻamaʻi
Centrifuge (aoina mai o sela), ultra-mama worktable, inverted microscope, CO2 incubator (incubating culture), vai taele, tolu-oxygen disinfection ma sterilization masini, 4 ℃ pusaaisa (tuuina serum ma aganuu fofo).
2. Fa'agaioiga aseptic
（1） Fa'amama ole potu mama
1. Fa'amama le potu fa'amama i taimi uma: fa'atasi i le vaiaso, fa'aaoga muamua le vai paipa e fufulu ai le fola, solo le laulau, ma fa'amama le laulau faigaluega, ona fa'aaoga lea o le 3 ‰ lysol po'o le bromogeramine po'o le 0.5% peracetic acid e solo ai.
2. Fa'amama o le CO2 incubator (incubator): muamua solo i le 3 ‰ bromogeramine, ona solo lea i le 75% alcohol po'o le 0.5% peracetic acid, ona fa'asusu lea i le moli ultraviolet.
3. Fa'amama a'o le'i fa'ata'ita'i: fa'amumu le moli ultraviolet, le tolu-oxygen sterilizer ma le fa'amama ea mo le 20-30 minute.
4. Fa'amama pe a uma le fa'ata'ita'iga: solo le laulau fa'amama ultra, laulau pito ma le fa'ata'ita'i fa'afo'i fa'asaga i le 75% ava malosi (3 ‰ bromogeramine).
Fa'amamago tapenaga o tagata faigaluega su'esu'e
1. Fufulu lima i le fasimoli.
2. Fai ofu tuufua, pulou tuuese, matapulepule ma seevae tosotoso.
3. Tape lima i le 75% ava malosi polo cotton.
Fa'aaliga ole fa'agaioiga mama
1. O fagu ava malosi uma, PBS, culture medium ma trypsin e aumai i totonu o le ultra-clean workbench e tatau ona solo i le 75% ava malosi ile pito i fafo ole fagu.
2. Fa'agaoioi i tafatafa o le mumu o le moli o le ava.
3. E tatau ona fa'amama a'o le'i fa'aogaina mea.
4. O meafaitino (e pei o tapuni fagu ma fa'a'a'ai) o lo'o fa'aauau pea ona fa'aoga e tatau ona tu'u i se mea maualuga, ma e tatau lava ona fa'aavela a'o fa'aoga.
5. O gaioiga uma e tatau ona latalata i le moli ava malosi, ma o le gaioiga e tatau ona mama ma saʻo, ma e le tatau ona paʻi faʻafuaseʻi.Afai e le mafai e le vaomago ona pa'i i le tane vai otaota.
6. Pe a aspirating sili atu ma le lua ituaiga o vai, faʻalogo lelei i le sui o le paipa suction e puipuia faʻafefe faʻafefe.
Va'ai le mataupu o lo'o soso'o mai mo le fa'amamago o mea faigaluega.
Taimi meli: Feb-01-2023